Contributors.- Abbreviations.- Forword.- Introduction.- Part 1. PCR Fundamentals.- 1: PCR Basics.- 2: PCR Methodology.- 3: Laboratory Accreditation Standards.- Part 2: Clinical Aspects and Diagnosis of Infectious Disease.- 4: Meningitis and Encephalitis.- 5: Respiratory Infections.- 6: Blood-borne Disease.- 7: Enteric Infections.- 8: Sexually Transmitted Infections.- Part 3: PCR Protocols for Bacterial Pathogens.- 9: Bordetella pertussis and Bordetella parapertussis.- 10: Brucellosis - the role of PCR in diagnosis and management.- 11: Burkholderia cepacia complex and Burkholderia gladioli.- 12: Chlamydia and Lymphogranuloma venereum (LGV).- 13: Chlamydophila.- 14: Coxiella burnetii.- 15: Diarrheagenic E coli Pathotypes (DEP) including Enterohaemorrhagic (EHEC)/Shiga-toxin E. coli (STEC).- 16: Haemophilus ducreyi and Klebsiella granulomatis.- 17: Haemophilus influenzae - capsulated and non-typeable.- 18: Mycobacterium tuberculosis complex.- 19: Mycobacteria Other Than Mycobacterium tuberculosi.- 20: Mycoplasma genitalium.- 21: Mycoplasma pneumoniae.- 22: Neisseria gonorrhoeae.- 23: Neisseria meningitides.- 24: Pseudomonas aeruginosa.- 25: Rickettsia.- 26: Streptococcus pneumoniae.- 27: Treponema pallidum.- 28: Universal Bacteria Identification by PCR and DNA sequencing of 16SrRNA gene.- Part 4: PCR Protocols for Viral Pathogens.- 29: Adenovirus.- 30: Cytomegalovirus (CMV).- 31: Dengue Virus.- 32: Enterovirus.- 33: Epstein Barr virus.- 34: Flavivirus.- 35: Hepatitis A virus.- 36: Hepatitis B virus.- 37: Hepatitis C virus.- 38: Hepatitis D virus.- 39: Hepatitis E virus.- 40: Herpes Simplex Virus Type 1 and 2.- 41: Human Bocavirus.- 42: Human Coronaviruses.- 43: Human Herpes Viruses -6, -7 and -8.- 44: Human Papillomavirus.- 45: Human Polyomaviruses - JCV and BKV.- 46: Human Polyomaviruses - KIV and WUV.- 47: Human Rhinoviruses.- 48: Influenza Virus A H5N1 ("Avian Influenza").- 49: Influenza Virus A H1N1(2009) ("Human Swine Influenza").- 50:Influenza Type C.- 51: Measles Virus.- 52: Norovirus.- 53: Respiratory Syncytial Virus Types A and B.- 54: Rotavirus.- 55: Varicella Zoster Virus.- 56: Eight Commonly Recognised Respiratory Viruses.- 57: Detection of CMV, HSV, VZV, EBV and Enterovirus by Multiplex PCR.- Part 5: PCR Protocols for Fungal and Parasitic Pathogens.- 58: Aspergillus Species.- 59: Cryptosporidium.- 60: Cyclospora.- 61: Dientamoeba fragilis.- 62: Entamoeba histolytica.- 63: Giardia.- 64: Malaria (P.falciparum, P.vivax, P.malariae, P.ovale).- 65: Microsporidia.- 66: Pneumocystis jirovecii.- 67: Trichomonas vaginalis.- 68: Toxoplasma gondii.- 69: Multiplex PCR for Protozoan Parasites.- 70: Universal Detection and Identification of Fungi by PCR and DNA Sequencing.- Part 6: Susceptibility Screening.- 71: An Introduction to Antimicrobial Susceptibility Screening.- 72: PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene.- 73: Detection of VRE : vanA and vanB genes by PCR.- 74: Metallo ? lactamases bla imp, blaspm and blavim Detection by Multiplex Real-time TaqMan Assay on the Smartcycler.- 75: PCR-Sequencing for Detection of Human Cytomegalovirus Mutations Conferring Antiviral Resistance.