PART I: THEORY
Chapter 1. The Basics
FFF Overview
Historical perspectives
Normal vs steric vs hyperlayer modes
Retention
Resolution, fractionation power
Chapter 2. FFF techniques
AF4 - Flow FFF (asymmetric, frit inlet and outlet)
HF5- Hollow-fiber flow FFF
Sedimentation FFF
Thermal FFF
Electrical FFF
Novel techniques (including 2D-FFF)
Chapter 3. User guide
Which FFF is right for you?
How to use FF correctly?
General optimization strategies: carrier fluid, calibration, reproducibility
Sample overloading
Sample recovery
Which detector to choose?
How to troubleshoot FFF? (in general terms)
Chapter 4. Other physical fractionation techniques: focus on performance as compared to the FFF
Filtration/Ultrafiltration
Centrifugation/ultracentrifugation
Size exclusion chromatography
Liquid chromatography
Microfluidics
Precipitation
 
PART II: APPLICATIONS
Chapter 5. Polymer and latex
Chapter 6. Biological particles: Virus, DNA/RNA, drug delivery
Chapter 7. Nanoparticles in water and soil
Chapter 8. Manufactured nanoparticles
Chapter 9. Protein, enzymes
Chapter 10. Cell separations - blood and algal cells
Chapter 11. Food industry - Starch
Chapter 12. Pharmaceutical industry - drug delivery
Chapter 13. Disease diagnosis